Journal: Scientific Reports

Article Title: In vitro complement activation via nucleocapsid and spike proteins of SARS-CoV-2 in COVID-19 patients

doi: 10.1038/s41598-025-20926-6

Figure Lengend Snippet: Comparison of the in vitro C3b and C4b complement depositions via spike (C3b(S) and C4b(S)) or nucleocapsid (C3b(N) and C4b(N)) recombinant proteins between patients with (Ab(+)) or without (Ab(-)) SARS-CoV-2-specific IgM and IgG antibodies from four different COVID-19 groups (CONV, HOSP, HOSP + O 2 and ICU). The in vitro C3b and C4b complement depositions were measured by our in-house complement deposition assays, where we used carrier free recombinant nucleocapsid or spike proteins and non-heat-inactivated patient sera. The p values for the pair-wise group comparisons on the violin plots were calculated by the Mann-Whitney tests. P values of significant differences are marked as p MW between without and with specific antibodies. Non-significant differences are marked as n.s.

Article Snippet: Mouse anti-human IgG1 Fc-HRP (HP6001) , SouthernBiotech , 9054-05.

Techniques: Comparison, In Vitro, Recombinant, MANN-WHITNEY

Journal: Scientific Reports

Article Title: In vitro complement activation via nucleocapsid and spike proteins of SARS-CoV-2 in COVID-19 patients

doi: 10.1038/s41598-025-20926-6

Figure Lengend Snippet: Comparison of the levels of SARS-CoV-2-specific IgM and IgG among four COVID-19 groups (CONV, HOSP, HOSP + O2, ICU) measured by Generic Assays CoV-2 IgM or IgG ( a ). The percentages of SARS-CoV-2-specific IgG or IgM positivity were shown also for four different COVID-19 groups. The numbers represent the mean percentages of SARS-CoV-2-specific IgM in blue or specific IgG in green circle sectors ( b ). The levels of total S- or N-specific IgG were quantified by in-house ELISA, normalized to positive and negative serum samples ( c ). The levels of S-specific and N-specific IgG1 ( d ) and IgG3 ( e ) subclasses were quantified by in-house ELISA calibrated with purified human IgG1 and IgG3. The p -values ( p KW ) were determined by Kruskal-Wallis test.

Article Snippet: Mouse anti-human IgG1 Fc-HRP (HP6001) , SouthernBiotech , 9054-05.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Purification

Journal: Scientific Reports

Article Title: In vitro complement activation via nucleocapsid and spike proteins of SARS-CoV-2 in COVID-19 patients

doi: 10.1038/s41598-025-20926-6

Figure Lengend Snippet: The in vitro C3b and C4b complement depositions via spike (C3b(S) and C4b(S)) recombinant protein are plotted by the levels of S-specific IgG1 and IgG3 in CONV individuals and in the other three hospitalized COVID-19 groups (HOSP, HOSP + O 2 and ICU) ( a ). The in vitro C3b and C4b complement depositions via nucleocapsid (C3b(N) and C4b(N)) recombinant protein are plotted by the levels of N-specific IgG1 and IgG3 in CONV individuals and in the other three hospitalized COVID-19 groups (HOSP, HOSP + O 2 and ICU) ( b ).

Article Snippet: Mouse anti-human IgG1 Fc-HRP (HP6001) , SouthernBiotech , 9054-05.

Techniques: In Vitro, Recombinant

Journal: Scientific Reports

Article Title: In vitro complement activation via nucleocapsid and spike proteins of SARS-CoV-2 in COVID-19 patients

doi: 10.1038/s41598-025-20926-6

Figure Lengend Snippet: Comparison of the levels of SARS-CoV-2-specific IgM and IgG antibodies, and in vivo complement profiles in all COVID-19 patients between low and high in vitro C3b(N) complement depositions. The p values for the pair-wise group comparisons on the violin plots were calculated by the Mann-Whitney tests. Asterisks indicate p value < 0.0143 , considered significant results after 5% FDR correction using the Benjamini-Hochberg method.

Article Snippet: Mouse anti-human IgG1 Fc-HRP (HP6001) , SouthernBiotech , 9054-05.

Techniques: Comparison, In Vivo, In Vitro, MANN-WHITNEY

Journal: PLOS ONE

Article Title: Crystalline silica-exposed human lung epithelial cells presented enhanced anchorage-independent growth with upregulated expression of BRD4 and EZH2 in autocrine and paracrine manners

doi: 10.1371/journal.pone.0285354

Figure Lengend Snippet: (A) ELISA of EGF, TNF-α, IL-6, and TGF-β1 was performed with the culture supernatants of nonadherent NL20, BEAS-2B, and 16HBE 4–6 days after incubation with autocrine CS or BPDE CM compared with the autocrine control CM. ELISA of EGF, TNF-α, and IL-6 was also conducted with the culture supernatants 48 h after exposure to CS in adherent bronchial cell lines. Asterisks indicate a significant difference in concentration (*p < 0.05, **p < 0.01). Relative values are shown as mean ± SD from three independent experiments. (B) Anchorage-independent growth of NL20, BEAS-2B, and 16HBE cells was measured using MTT 5–6 days after the administration of recombinant human EGF, TNF-α, and IL-6. The MTT assay was performed for autocrine CS CM-incubated nonadherent NL20 and BEAS-2B cells four and five days after EGF-neutralizing antibody (NtAb) treatment, respectively, and for autocrine CS CM-incubated nonadherent 16HBE cells, five days after TNF-α NtAb treatment. The MTT assay was also performed for autocrine CS CM-incubated nonadherent bronchial cell lines five days after IL-6 NtAb administration. Asterisks indicate a significant difference in growth (*p < 0.05, **p < 0.01). Relative values are shown as mean ± SD from three independent experiments. (C) Expression of BRD4 and EZH2 in nonadherent 16HBE cells three days after the administration of recombinant human TNF-α determined using western blotting and the expression of BRD4 and EZH2 3–4 days after treatment of nonadherent NL20 and BEAS-2B cells with recombinant human EGF.

Article Snippet: Mouse anti-human EGF neutralizing antibody (NtAb), goat anti-human TNF-α NtAb, and mouse anti-human IL-6 NtAb were obtained from R&D Systems.

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Control, Concentration Assay, Recombinant, MTT Assay, Expressing, Western Blot

Journal: JAMA Neurology

Article Title: Association of MOG-IgG Serostatus With Relapse After Acute Disseminated Encephalomyelitis and Proposed Diagnostic Criteria for MOG-IgG–Associated Disorders

doi: 10.1001/jamaneurol.2018.1814

Figure Lengend Snippet: Clinical and Serological Characteristics of Pediatric and Adult Patients With Acute Disseminated Encephalomyelitis (ADEM)

Article Snippet: The median fluorescence intensity-bound Alexa Fluor 647–conjugated mouse antihuman IgG1 (Fc region–specific; Catalog No. 9054-01; Southern Biotech) was determined for both nontransfected and transfected cells.

Techniques: Infection

Journal: The Journal of infectious diseases

Article Title: Chemotherapy accelerates the development of acquired immune responses to Schistosoma haematobium infection.

doi: 10.1086/517456

Figure Lengend Snippet: Figure 1. Patterns of infection intensities and antibody levels. A, Mean ({SE) log(x / 1)-transformed S. haematobium egg counts/10 mL of urine by age group in study population before treatment. Sam- ple sizes by age group were 13, 59, 108, 20, 17, 21, 15, 14, and 26, respectively (total, 293 subjects; 151 female, 142 male). B, Mean ({SE) of square root–transformed absorbances at 492 nm for anti– soluble egg antigen (SEA)–specific IgA (l) and IgG1 (j) by age group in study population before treatment. Samples sizes by age group for IgA were 0, 49, 98, 19, 15, 19, 14, 12, and 25, respectively (total, 251 subjects; 128 female, 123 male). Sample sizes by age group for IgG1 were 0, 42, 84, 18, 15, 19, 14, 12, and 25, respectively (total, 229 subjects; 115 female, 114 male). C, Mean ({SE) of square root–transformed absorbances for anti-SEA–specific IgA (l) and IgG1 (j) in children who had positive egg counts before treatment. Rx Å treatment. Sample sizes by time point for IgA were 41 (20 female, 21 male) before treatment and 16, 38, 32, 26, and 27, respec- tively, thereafter. Sample sizes by time point for IgG1 were 40 (20 female, 20 male) before treatment and 16, 38, 32, 26, and 27, respec- tively, thereafter.

Article Snippet: That is, high levels of IgA withinwas added at 1:2000 dilution (using horseradish peroxidase–conjuage groups were associated with infection but high levels ofgated mouse anti–human IgG1 (MCA514P; Serotec, Oxford, UK) IgG1 were not, although levels of the two antibodies wereand goat anti–human IgA (A-7032; Sigma, St. Louis).

Techniques: Infection, Transformation Assay

Journal: The Journal of infectious diseases

Article Title: Chemotherapy accelerates the development of acquired immune responses to Schistosoma haematobium infection.

doi: 10.1086/517456

Figure Lengend Snippet: Figure 2. Variations in levels of anti–S. haematobium soluble egg antigen– specific IgA and IgG1. A, Children 5–16 years old before treatment showing egg-positive (j or h) and egg-negative (L) individuals and members of cohort (j); n Å 133 from 187 possible. B, Adults ú16 years old before treatment; n Å 85 from 93 possible. h Å egg-positive, L Å egg-negative. C, Egg-positive children 18 weeks after treatment with praziquantel; n Å 38 from 40 possible. Compare with js in A.

Article Snippet: That is, high levels of IgA withinwas added at 1:2000 dilution (using horseradish peroxidase–conjuage groups were associated with infection but high levels ofgated mouse anti–human IgG1 (MCA514P; Serotec, Oxford, UK) IgG1 were not, although levels of the two antibodies wereand goat anti–human IgA (A-7032; Sigma, St. Louis).

Techniques: